However, the GB squad go into this match buoyed by their performances in Turkey which saw the Brits win two of their three games and finish runners-up in the International Foundation Tournament.
GB lost their final game to Turkey on Sunday, but enjoyed great victories over world number 9 ranked, South Korea and Argentina who are ranked 12th.
For the Czechs, it will be their final preparation before they head to Ankara, Turkey for next week's Olympic Qualifying Tournament.
When Great Britain take to the floor on Tuesday, it will be exactly a year to the day since they last faced the Czech Republic.
On that occasion it was in the group stages of EuroBasket and the Czechs ran out 60-45 winners, with shooting guard Katerina Elhotova leading the way with 27 points.
GB's top scorer that night was Jo Leedham (14 points) and Leedham will go into the Prague games on a good run of form, having won the Most Valuable Player award in Ankara at the weekend, after averaging 21.7 points over the three games.
The Czechs finished fourth at EuroBasket Women Finals 2011 and won the silver medal at the 2010 World Championships, but GB Coach Tom Maher is looking forward to the chance to see how his improving side fares against one of the world's best teams: "The Czech Republic have always been a top six team," said Maher. "Recently second in the World Championships and a very tall side, with very fine shooters.
"It'll be a fine test for us and we look forward to it."
The games will take place in Prague with a 5pm (BST) tip-off on both Tuesday and Wednesday.