The Czechs responded with a 9-0 run to take the lead back up to double figures (54-42) and although GB would get no closer than eight points the rest of the way, they will be able to take a lot of positives from this game.
British Basketball Performance Director, Chris Spice, praised the effort shown: "We competed really well with a world-class team, used our bench and everyone played some good minutes.
"We gave a lot of good minutes to the youngsters and they held up very well against quality opposition."
Spice added: "The first quarter we were slow to start and didn't really recover from then but we pushed them all the way, so we're very pleased with a good work-rate and good effort by everyone."
Jo Leedham led all scorers with 13 points, while Rose Anderson impressed with 10 points and four rebounds in 13 minutes of court time.
Guard Natalie Stafford believes GB have a chance to win Wednesday's re-match: "We're very happy with today's performance against the Czech Republic. We gave them a run for their money and got things going defensively.
"I think if we had just made some shots, it would have been a different story and a much closer game."
"We're looking forward to the game tomorrow," she added. "We've realised we can hang with them and give them a run for their money, so it will be a much better performance tomorrow."
Great Britain will seek to get that win against the Czech Republic when the two sides meet again in Prague on Wednesday, tip-off 5pm (BST).
For box score click here.
